Method 8081B: Organochlorine Pesticides Analysis by GC (Extended)

Many labs extend the list of compounds tested using Method 8081B to include other organochlorine pesticides. Rtx-CLPesticides and Rtx-CLPesticides2 columns are ideal for these determinations and can be used in a parallel dual-column configuration to reliably quantify 38 commonly analyzed organochlorine pesticides, as well as many other compounds. Full separations are achieved between both columns and the unique, optimized selectivity allows additional compounds to be included. (Note that since extended compound lists vary among laboratories the lists compared here are not identical.)

Comparison based on published competitor data. All columns tested were 0.32 mm ID. NDP = no data published

	Peaks	Conc. (wt.%)
1.	1,2-Dibromo-3-chloropropane	100
2.	Hexachloro-cyclopentadiene	100
3.	Tetrachloro-m-xylene (SS)	100
4.	cis-Diallate	500
5.	Hexachlorobenzene	100
6.	trans-Diallate	500
7.	α-BHC	40
8.	γ-BHC	40
9.	β-BHC	40
10.	δ-BHC	40
11.	Heptachlor	40
12.	Aldrin	40

	Peaks	Conc. (wt.%)
13.	Chlorpyrifos	100
14.	Isodrin	100
15.	Oxychlordane	50
16.	Heptachlor epoxide	40
17.	trans-Chlordane	40
18.	2,4'-DDE	100
19.	trans-Nonachlor	100
20.	cis-Chlordane	40
21.	Endosulfan I	40
22.	Captan	100
23.	4,4'-DDE	100
24.	Dieldrin	80

	Peaks	Conc. (wt.%)
25.	2,4'-DDD	100
26.	Endrin	80
27.	Chlorobenzilate	500
28.	2,4'-DDT	100
29.	cis-Nonachlor	100
30.	4,4'-DDD	80
31.	Endosulfan II	80
32.	4,4'-DDT	80
33.	Endrin aldehyde	80
34.	Endosulfan sulfate	80
35.	Methoxychlor	400
36.	Mirex	100
37.	Endrin ketone	80
38.	Decachloro-biphenyl	100

Columns	Rtx-CLPesticides2 30 m, 0.32 mm ID, 0.25 µm (cat.# 11324)
	and Rtx-CLPesticides 30 m, 0.32 mm ID, 0.32 µm (cat.# 11141)
	using Rxi guard column 5 m, 0.32 mm ID (cat.# 10039)
	with deactivated universal “Y” Press-Tight connector (cat.# 20405-261)
Standard/Sample	Organochlorine pesticide mix AB #2 (cat.# 32292)
	Organochlorine pesticide mix C #1 (cat.# 32296)
	2,4,5,6-Tetrachloro-m-xylene (cat.# 32027)
	Decachlorobiphenyl (BZ #209) (cat.# 32029)
	Chlorpyrifos (cat.# 32212)
	2,4'-DDE (cat.# 32099)
	2,4'-DDD (cat.# 32098)
	Chlorobenzilate (cat.# 32211)
	Captan (custom)
	cis-Nonachlor (custom)
	Mirex (custom)
	Oxychlordone (custom)
	Diallate (custom)
Diluent:	Hexane
Injection
Inj. Vol.:	1 µL splitless (hold 0.3 min)
Liner:	Cyclo double taper (4 mm) (cat.# 20896)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	80 °C to 175 °C at 8 °C/min to 195 °C at 11 °C/min to 235 °C at 7 °C/min to 320 °C at 25 °C/min (hold 3 min)
Carrier Gas	He, constant flow
Linear Velocity:	28 cm/sec

Detector	Micro-ECD @ 330 °C
Make-up Gas Flow Rate:	60 mL/min
Make-up Gas Type:	N2
Instrument	Agilent/HP6890 GC
Notes	This chromatogram was obtained using an Agilent micro-ECD. To obtain comparable results, you will need to employ a micro-ECD in addition to confirmational dual columns connected to a 5-meter guard column using a “Y” Press-Tight connector.

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